Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. [artículo]
Por: Castillo Rama, Marcela [Inmunología]
| Castro Panete, María José [Inmunología] | Meneu Díaz, Juan Carlos [Cirugía General y del Aparato Digestivo] | Mora Diaz, Sergio [Inmunología] | Morales Pérez, Pablo [Inmunología] | Moreno González, Enrique [Cirugía General y del Aparato Digestivo] | Paz Artal, Estela [Inmunología] | Talayero Giménez de Azcárate, Paloma [Inmunología] | Valero Hervás, Diana María [Inmunología].
Colaborador(es): Servicio de Inmunología | Servicio de Cirugía General y del Aparato Digestivo | Instituto de Investigación imas12.
Editor: European Journal of Inflammation, 2012Descripción: 10(3):329-34.Recursos en línea: Solicitar documento Resumen: "Slow" and "Fast" C3 complement variants (C3S and C3F) result from a g.304C>G polymorphism that changes arginine to glycine at position 102. C3 variants are associated with complement-mediated diseases and outcome in transplantation. In this work C3 genotyping is achieved by a Real Time PCR - High Resolution Melting (RT-PCR-HRM) optimized method. In an analysis of 49 subjects, 10.2% were C3FF, 36.7% were C3SF and 53.1% were C3SS. AUelic frequencies (70% for C3S and 30% for C3F) were in Hardy-Weinberg equilibrium and similar to those published previously. When comparing RT-PCR-HRM with the currently used Tetraprimer-Amplification Refractory Mutation System PCR (T-ARMS-PCR), coincidence was 93.8"/o. The procedure shown here includes a single primer pair and
low DNA amount per reaction. Detection of C3 variants by RT-PCR-HRM is accurate, easy, fast and low cost, and it may be the method of choice for C3 genotyping.
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Artículo
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Formato Vancouver:
Valero-Hervás DM, Morales P, Castro MJ, Varela P, Castillo-Rama M, Moreno E et al. Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. Eur J Inflamm. 2012;10(3):329-34.
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"Slow" and "Fast" C3 complement variants (C3S and C3F) result from a g.304C>G polymorphism that changes arginine to glycine at position 102. C3 variants are associated with complement-mediated diseases and outcome in transplantation. In this work C3 genotyping is achieved by a Real Time PCR - High Resolution Melting (RT-PCR-HRM) optimized method. In an analysis of 49 subjects, 10.2% were C3FF, 36.7% were C3SF and 53.1% were C3SS. AUelic frequencies (70% for C3S and 30% for C3F) were in Hardy-Weinberg equilibrium and similar to those published previously. When comparing RT-PCR-HRM with the currently used Tetraprimer-Amplification Refractory Mutation System PCR (T-ARMS-PCR), coincidence was 93.8"/o. The procedure shown here includes a single primer pair and
low DNA amount per reaction. Detection of C3 variants by RT-PCR-HRM is accurate, easy, fast and low cost, and it may be the method of choice for C3 genotyping.
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