Castillo Rama, Marcela Castro Panete, María José Meneu Díaz, Juan Carlos Mora Diaz, Sergio Morales Pérez, Pablo Moreno González, Enrique Paz Artal, Estela Talayero Giménez de Azcárate, Paloma Valero Hervás, Diana María
Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. [artículo] - European Journal of Inflammation, 2012 - 10(3):329-34.
Formato Vancouver:
Valero-Hervás DM, Morales P, Castro MJ, Varela P, Castillo-Rama M, Moreno E et al. Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. Eur J Inflamm. 2012;10(3):329-34.
Contiene 16 referencias
"Slow" and "Fast" C3 complement variants (C3S and C3F) result from a g.304C>G polymorphism that changes arginine to glycine at position 102. C3 variants are associated with complement-mediated diseases and outcome in transplantation. In this work C3 genotyping is achieved by a Real Time PCR - High Resolution Melting (RT-PCR-HRM) optimized method. In an analysis of 49 subjects, 10.2% were C3FF, 36.7% were C3SF and 53.1% were C3SS. AUelic frequencies (70% for C3S and 30% for C3F) were in Hardy-Weinberg equilibrium and similar to those published previously. When comparing RT-PCR-HRM with the currently used Tetraprimer-Amplification Refractory Mutation System PCR (T-ARMS-PCR), coincidence was 93.8"/o. The procedure shown here includes a single primer pair and
low DNA amount per reaction. Detection of C3 variants by RT-PCR-HRM is accurate, easy, fast and low cost, and it may be the method of choice for C3 genotyping.
Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. [artículo] - European Journal of Inflammation, 2012 - 10(3):329-34.
Formato Vancouver:
Valero-Hervás DM, Morales P, Castro MJ, Varela P, Castillo-Rama M, Moreno E et al. Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. Eur J Inflamm. 2012;10(3):329-34.
Contiene 16 referencias
"Slow" and "Fast" C3 complement variants (C3S and C3F) result from a g.304C>G polymorphism that changes arginine to glycine at position 102. C3 variants are associated with complement-mediated diseases and outcome in transplantation. In this work C3 genotyping is achieved by a Real Time PCR - High Resolution Melting (RT-PCR-HRM) optimized method. In an analysis of 49 subjects, 10.2% were C3FF, 36.7% were C3SF and 53.1% were C3SS. AUelic frequencies (70% for C3S and 30% for C3F) were in Hardy-Weinberg equilibrium and similar to those published previously. When comparing RT-PCR-HRM with the currently used Tetraprimer-Amplification Refractory Mutation System PCR (T-ARMS-PCR), coincidence was 93.8"/o. The procedure shown here includes a single primer pair and
low DNA amount per reaction. Detection of C3 variants by RT-PCR-HRM is accurate, easy, fast and low cost, and it may be the method of choice for C3 genotyping.
