000			03124na a2200337 4500
003			H12O
005			20210702062700.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_91016 _aDelgado Vázquez, Rafael _eMicrobiología y Parasitología
100			_aFiorante, Silvana _91609 _eMedicina Interna
100			_aLuczkowiak, Joanna _91611 _eMicrobiología y Parasitología
100			_91612 _aMartínez Prats, Lorena _eMicrobiología y Parasitología
100			_9868 _aRodríguez Otero, Joaquín _eMicrobiología y Parasitología
100			_91026 _aPulido Ortega, Federico _eUnidad VIH
100			_91500 _aRubio García, Rafael _eUnidad de VIH
100			_959 _aOlalla Sierra, Julián _eMedicina Interna
245	0	0	_aLack of the Detection of XMRV or Polytropic MLV-Related Sequences in Blood Cells From HIV-1–Infected Patients in Spain. _h[artículo]
260			_bJAIDS Journal of Acquired Immune Deficiency Syndromes, _c2012
300			_a59(2):101-4.
500			_aFormato Vancouver: Luczkowiak J, Martínez-Prats L, Sierra O, Fiorante S, Rubio R, Pulido F, et al. Lack of the detection of XMRV or polytropic MLV-related sequences in blood cells from HIV-1-infected patients in Spain. J Acquir Immune Defic Syndr. 2012 Feb 1;59(2):101-4.
501			_aPMID:21963942
504			_aContiene 24 referencias
520			_aXenotropic murine leukemia virus-related virus (XMRV) and polytropic murine leukemia virus (MLV)-related virus are recently described human gammaretroviruses that have been associated with prostate cancer and chronic fatigue syndrome. These studies have been controversial because a number of laboratories have been unable to find evidence of XMRV in similar groups of patients or controls. Because the existence of XMRV raises many questions, we decided to study its presence in a group of patients infected with HIV-1 with a high proportion of intravenous drug use and coinfection by hepatitis C virus. METHODS: Forty HIV-1-infected patients under follow-up in our institution were screened for XMRV/MLV by nested polymerase chain reaction using primers targeting the gag and env region. Specific primers for mouse mitochondrial DNA were used to rule out contamination. RESULTS: No evidence of XMRV or polytropic MLV-related sequences was found in any sample from patients or controls. Four samples yielded polymerase chain reaction bands whose sequence corresponded to murine endogenous retroviral sequences, however, contamination with mouse cell DNA was subsequently confirmed. CONCLUSIONS: XMRV/MLV viruses do not seem to be associated with HIV-1 infection or intravenous drug use. Contamination of samples or reagents by genomic murine DNA or XMRV vectors could account for the sporadic detection of positive samples for XMRV and related agents.
710			_96 _aServicio de Medicina Interna
710			_981 _aServicio de Microbiología y Parasitología
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/8/pc8396.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c8396 _d8396