000			02818nam a2200277 4500
003			H12O
005			20180417112625.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_91105 _aGrau Sanz, Montserrat _eCirugía General y del Aparato Digestivo
100			_9510 _aMoreno González, Enrique _eCirugía General y del Aparato Digestivo
100			_aPaz Artal, Estela _91510 _eInmunología
100			_9661 _aSerrano Hernández, Antonio _eInmunología
245	0	0	_aBlockade of endothelial G(i) protein enhances early engraftment in intraportal cell transplant to mouse liver. _h[artículo]
260			_bCell Transplantation, _c2012
300			_a21(7):1383-96.
500			_aFormato Vancouver: Alfaro J, Grau M, Serrano M, Checa AI, Criado LM, Moreno E, et al. Blockade of endothelial G(i) protein enhances early engraftment in intraportal cell transplant to mouse liver. Cell Transplant. 2012;21(7):1383-96.
501			_aPMID: 22525519
504			_aContiene 66 referencias
520			_aThe limited availability of liver donors and recent progress in cell therapy technologies has centered interest on cell transplantation as a therapeutic alternative to orthotopic liver transplant for restoring liver function. Following transplant by intraportal perfusion, the main obstacle to cell integration in the parenchyma is the endothelial barrier. Transplanted cells form emboli in the portal branches, inducing ischemia and reperfusion injury, which cause disruption of endothelial impermeability and activate the immune system. Approximately 95% of transplanted cells fail to implant and die within hours by anoikis or are destroyed by the host immune system. Intravascular perfusion of Bordetella pertussis toxin (PTx) blocks endothelial G(i) proteins and acts as a reversible inducer of actin cytoskeleton reorganization, leading to interruption of cell confluence in vitro and increased vascular permeability in vivo. PTx treatment of the murine portal vascular tree 2 h before intraportal perfusion of embryonic stem cells facilitated rapid cell engraftment. By 2 h postperfusion, the number of implanted cells in treated mice was more than fivefold greater than in untreated controls, a difference that was maintained to at least 30 days posttransplant. We conclude that prior to cell transplant, PTx blockade of the G(i) protein pathway in liver endothelium promotes rapid, efficient cell implantation in liver parenchyma, and blocks chemokine receptor signaling, an essential step in early activation of the immune system.
710			_9271 _aServicio de Cirugía General y del Aparato Digestivo
710			_9395 _aServicio de Inmunología
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/7/pc7470.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c7470 _d7470