000			02363na a2200253 4500
003			H12O
005			20180417112625.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_9614 _aCabanillas Martín, Beatriz _eAlergología
100			_9617 _aFernández Crespo, Jesús _eAlergología
100			_9616 _aRodríguez Rodríguez, Julia _eAlergología
245	0	0	_aPine nut allergy: Clinical features and major allergens characterization _h[artículo]
260			_bMolecular Nutrition & Food Research, _c2012
300			_a56(12):1884-1893.
500			_aFormato Vancouver: Cabanillas B, Cheng H, Grimm CC, Hurlburt BK, Rodríguez J, Crespo JF, et al. Pine nut allergy: clinical features and major allergens characterization. Mol Nutr Food Res. 2012;56(12):1884-93.
501			_aPMID: 23081934
504			_aContiene 26 referencias
520			_aThe aims of this study were to evaluate IgE-mediated hypersensitivity to pine nut with details of clinical reactions and to characterize major pine nut allergens. METHODS AND RESULTS: The study included ten consecutive teenagers and adults diagnosed with IgE-mediated clinical allergy to pine nut. Two major pine nut allergens were purified and identified and the secondary structures and susceptibility to digestion were characterized. Severe reactions represent 80% of allergic reactions to pine nut in this study. Moreover, 70% of the patients were monosensitized to this nut. Two major allergens with molecular weights of 6 and 50 kDa were purified and identified as albumin and vicilin, respectively. The 6 kDa protein (albumin), rich in α-helix content, was far more stable to peptic and tryptic digestion as compared with 50 kDa protein (vicilin), which was quickly broken down. The secondary structure of the purified 50 kDa protein showed 41% β-sheet, 5% α-helix, and 54% random coil and/or loops. CONCLUSION: Eighty percent of allergic reactions to pine nut in the ten patients included in this study were severe. Most patients (70%) were monosensitized to this nut. Two major allergens with molecular weights of 6 and 50 kDa were purified and identified as albumin and vicilin, respectively.
710			_999 _aServicio de Alergología
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/7/pc7464.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c7464 _d7464