000			03132na a2200289 4500
003			H12O
005			20210625062803.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aCriado Carrasco, Gabriel _91880 _eInstituto de Investigación i+12
100			_aRey Cerros, Manuel José del _91881 _eInstituto de Investigación i+12
100			_aIzquierdo Alvárez, Elena _91882 _eInstituto de Investigación i+12
100			_91010 _aPablos Álvarez, José Luis _eReumatología
100			_aUsátegui Corral, Alicia _91883 _eInstituto de Investigación i+12
245	0	0	_aTranscriptome analysis reveals specific changes in osteoarthritis synovial fibroblasts. _h[artículo]
260			_bAnnals of the Rheumatic Diseases, _c2012
300			_a71(2):275-80.
500			_aFormato Vancouver: Del Rey MJ, Usategui A, Izquierdo E, Cañete JD, Blanco FJ, Criado G, et al. Transcriptome analysis reveals specific changes in osteoarthritis synovial fibroblasts. Ann Rheum Dis. 2012 Feb;71(2):275-80.
501			_aPMID: 22021863
504			_aContiene 42 referencias
520			_aObjective Changes in rheumatoid arthritis synovial fibroblast (RASF) gene expression are usually defined by a comparison to osteoarthritis synovial fibroblasts (OASFs). This study was undertaken to analyse the transcriptome of OASFs as compared to RASFs and healthy synovial fibroblasts (HSFs). Methods The authors used microarray messenger RNA expression profiling of synovial fibroblasts cultured from osteoarthritis (OA), rheumatoid arthritis and normal synovial tissues. Quantitative real-time PCR of selected genes was performed to validate microarray data. Analysis of variance, Student t test and the Benjamini-Hochberg multiple testing correction method for multiple testing correction were used to determine the statistical significance of the changes between the three groups. Results Larger numbers of transcripts showed a differential expression in OASFs versus the other groups, rather than in RASFs versus HSFs. Cluster analysis confirmed that the differences between the three groups were mostly due to the differences between OA and the other groups. Functional classification identified a significant number of genes related to growth factor activities, cell adhesion, neurotransmission and Ras signalling that are differentially expressed in OASFs. Classical proinflammatory factors or proteases involved in cartilage degradation were not found to be overexpressed in OASFs. Conclusion Cultured OASFs display a more homogeneous transcriptomic profile than RASFs when compared to HSFs. This supports the participation of synovial fibroblasts in the pathogenesis of OA and may reflect global defects in the mesenchyma-derived lineages of the different tissues in OA joints. These data support individual heterogeneity among RASFs and advise against the use of OASFs as controls.
710			_9123 _aServicio de Reumatología
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/6/pc6224.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c6224 _d6224