000			03190na a2200301 4500
003			PC6158
005			20210625062803.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_92247 _aSánchez Pérez, Ricardo _eInstituto de Investigación i+12
100			_91206 _aAyala Díaz, Rosa María _eHematología y Hemoterapia
100			_91473 _aAlonso Fernández, Rafael Alberto _eHematología
100			_9732 _aMartínez Sánchez, María Pilar _eHematología y Hemoterapia
100			_aSánchez Pina, José María _92248 _eHematología y Hemoterapia
100			_9389 _aMartínez López, Joaquín _eHematología y Hemoterapia
245	0	0	_aClinical characteristics of patients with central nervous system relapse in BCR-ABL1-positive acute lymphoblastic leukemia: the importance of characterizing ABL1 mutations in cerebrospinal fluid. _h[artículo]
260			_bAnnals of Hematology, _c2017
300			_a96(7):1069-1075.
500			_aFormato Vancouver: Sánchez R, Ayala R, Alonso RA, Martínez MP, Ribera J, García O et al. Clinical characteristics of patients with central nervous system relapse in BCR-ABL1-positive acute lymphoblastic leukemia: the importance of characterizing ABL1 mutations in cerebrospinal fluid. Ann Hematol. 2017 Jul;96(7):1069-1075.
501			_aPMID: 28451802
504			_aContiene 21 referencias
520			_aAs the search for an effective human malaria vaccine continues, understanding immune responses to Plasmodium in rodent models is perhaps the key to unlocking new vaccine strategies. The recruitment of parasite-specific antibodies is an important component of natural immunity against infection in blood-stage malaria. Here, we describe the use of sera from naturally surviving ICR mice after infection with lethal doses of Plasmodium yoelii yoelii 17XL to identify highly immunogenic blood-stage antigens. Immobilized protein A/G was used for the affinity-chromatography purification of the IgGs present in pooled sera from surviving mice. These protective IgGs, covalently immobilized on agarose columns, were then used to isolate reactive antigens from whole P. yoelii yoelii 17XL protein extracts obtained from the blood-stage malaria infection. Through proteomics analysis of the recovered parasite antigens, we were able to identify two endoplasmic reticulum lumen proteins: protein disulfide isomerase and a member of the heat shock protein 70 family. Also identified were the digestive protease plasmepsin and the 39 kDa-subunit of eukaryotic translation initiation factor 3, a ribosome associated protein. Of these four proteins, three have not been previously identified as antigenic during blood-stage malaria infection. This procedure of isolating and identifying parasite antigens using serum IgGs from malaria-protected individuals could be a novel strategy for the development of multi-antigen-based vaccine therapies. (c) 2012 Elsevier GmbH. All rights reserved.
710			_9297 _aServicio de Hematología y Hemoterapia
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/6/pc6158.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c6158 _d6158