000 03360na a2200301 4500
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008 130622s2011 xxx||||| |||| 00| 0 eng d
040 _cH12O
041 _aeng
100 _aCriado Carrasco, Gabriel
_91880
_eInstituto de Investigación i+12
100 _aRey Cerros, Manuel José del
_91881
_eInstituto de Investigación i+12
100 _aIzquierdo Alvárez, Elena
_91882
_eInstituto de Investigación i+12
100 _91010
_aPablos Álvarez, José Luis
_eReumatología
100 _aUsátegui Corral, Alicia
_91883
_eInstituto de Investigación i+12
245 0 0 _aSynovial fibroblast hyperplasia in rheumatoid arthritis: clinicopathologic correlations and partial reversal by anti-tumor necrosis factor therapy
_h[artículo]
260 _bArthritis and Rheumatism,
_c2011
300 _a63(9):2575-2583.
500 _aFormato Vancouver: Izquierdo E, Cañete JD, Celis R, Del Rey MJ, Usategui A, Marsal S, et al. Synovial fibroblast hyperplasia in rheumatoid arthritis: clinicopathologic correlations and partial reversal by anti-tumor necrosis factor therapy. Arthritis Rheum. 2011;63(9):2575-83.
501 _aPMID: 21547893
504 _aContiene 36 referencias
520 _aSynovial fibroblast (SF) hyperplasia contributes to the pathogenesis of rheumatoid arthritis (RA), but quantitative information on this process is scarce. This study was undertaken to evaluate the fibroblast-specific marker Hsp47 as a quantitative marker for SFs and to analyze its clinicopathologic correlates and evolution after anti-tumor necrosis factor α (anti-TNFα) therapy. METHODS: Synovial biopsy samples were obtained from 48 patients with RA and 20 controls who were healthy or had osteoarthritis (OA). Twenty-five RA patients who had active disease at the time of biopsy underwent a second biopsy after anti-TNFα therapy. Immunolabeling for Hsp47, inflammatory cells, and vascular cell markers was performed. Hsp47-positive lining and sublining fractional areas were quantified, and their correlation with clinicopathologic variables was analyzed. RESULTS: In normal and diseased synovial tissue, Hsp47 was specifically and uniformly expressed by lining, sublining, and perivascular fibroblasts. Lining SF area was significantly increased in both RA and late OA tissue compared to normal tissue. Sublining SF area was increased in RA tissue but not in late OA tissue compared to normal tissue. Lining SF area was positively correlated with macrophage density, Disease Activity Score in 28 joints, and RA disease duration. In contrast, sublining SF area was negatively correlated with RA disease duration and activity. A significant reduction in lining SF area but not sublining SF area was observed after anti-TNFα therapy. CONCLUSION: Our findings indicate that Hsp47 is a reliable marker for quantifying SFs in human synovial tissue. Our data suggest that lining and sublining SFs undergo different dynamics during the course of the disease. Lining SF expansion parallels the activity and temporal progression of RA and can be partially reversed by anti-TNFα therapy.
650 _aAdult
710 _9625
_aInstituto de Investigación imas12
710 _9123
_aServicio de Reumatología
856 _uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/5/pc5805.pdf
_ySolicitar documento
942 _n0
_2ddc
_cART
999 _c5805
_d5805