000			na a22 4500
999			_c4807 _d4807
003			PC4807
005			20200129130046.0
008			130622s2013 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aRuiz Contreras, Jesús _9811 _ePediatría
245	0	0	_aRapid diagnosis of invasive pneumococcal disease in pediatric population. _h[artículo]
260			_bJournal of microbiological methods, _c2013
300			_a93(2):116-20.
500			_aFormato Vancouver: Picazo JJ, Contreras JR, Ríos E, Culebras E, Rodríguez-Avial I, Méndez C et al. Heracles Study Group. Rapid diagnosis of invasive pneumococcal disease in pediatric population. J Microbiol Methods. 2013 May;93(2):116-20.
501			_aPMID: 23499921
504			_aContiene 20 referencias
520			_aThe aim of this study was to evaluate the Binax NOW immunochromatographic pneumococcal antigen test for the identification of Streptococcus pneumoniae in pleural and cerebrospinal fluids from children with suspected invasive pneumococcal disease. The results were compared with those obtained by PCR. Binax NOW was applied to these samples as recommended by the manufacturer for urine and cerebrospinal samples. Detection of pneumococcal DNA was performed by real-time PCR assay targeting the autolysin gene (lytA). Of the 199 samples analyzed, 131 were positive by both Binax NOW and lytA PCR, and 36 samples were negative by both techniques. Using the real-time PCR as a comparative method to the Binax for the detection of S. pneumoniae, the sensitivity and specificity of Binax NOW was 88% and 72.5%, respectively. Of the 145 positive samples analyzed by Binax NOW, 119 showed intense coloring of the sample line and 26 showed weak intensity. Conventional culture is the most common method in clinical settings, but Binax NOW is an easier and faster test for identifying S. pneumoniae in pleural and cerebrospinal fluids from children with suspected invasive pneumococcal disease.
710			_9446 _aServicio de Pediatría-Neonatología
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/4/pc4807.pdf _ySolicitar documento
942			_n0 _2ddc _cART