000			02691na a2200277 4500
003			PC4556
005			20210625062801.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aAtencia Cibreiro, María Gabriela _92131 _eInstituto de Investigación i+12
100			_aCordero Vázquez, Pilar _92132 _eNeurología
100			_aEsteban Pérez, Jesús _92075 _eNeurología
100			_aGarcía Redondo, Alberto _91899 _eInstituto de Investigación i+12
245	0	0	_aGenetic biomarkers for ALS disease in transgenic SOD1(G93A) mice _h[artículo]
260			_bPloS ONE, _c2012
300			_a7(3):e32632.
500			_aFormato Vancouver: Calvo AC, Manzano R, Atencia-Cibreiro G, Oliván S, Muñoz MJ, Zaragoza P et al. Genetic biomarkers for ALS disease in transgenic SOD1(G93A) mice. PLoS One. 2012;7(3):e32632.
501			_aPMID: 22412900
504			_aContiene 64 referencias
520			_aThe pathophysiological mechanisms of both familial and sporadic Amyotrophic Lateral Sclerosis (ALS) are unknown, although growing evidence suggests that skeletal muscle tissue is a primary target of ALS toxicity. Skeletal muscle biopsies were performed on transgenic SOD1(G93A) mice, a mouse model of ALS, to determine genetic biomarkers of disease longevity. Mice were anesthetized with isoflurane, and three biopsy samples were obtained per animal at the three main stages of the disease. Transcriptional expression levels of seventeen genes, Ankrd1, Calm1, Col19a1, Fbxo32, Gsr, Impa1, Mef2c, Mt2, Myf5, Myod1, Myog, Nnt, Nogo A, Pax7, Rrad, Sln and Snx10, were tested in each muscle biopsy sample. Total RNA was extracted using TRIzol Reagent according to the manufacturer's protocol, and variations in gene expression were assayed by real-time PCR for all of the samples. The Pearson correlation coefficient was used to determine the linear correlation between transcriptional expression levels throughout disease progression and longevity. Consistent with the results obtained from total skeletal muscle of transgenic SOD1(G93A) mice and 74-day-old denervated mice, five genes (Mef2c, Gsr, Col19a1, Calm1 and Snx10) could be considered potential genetic biomarkers of longevity in transgenic SOD1(G93A) mice. These results are important because they may lead to the exploration of previously unexamined tissues in the search for new disease biomarkers and even to the application of these findings in human studies.
710			_9625 _aInstituto de Investigación imas12
710			_9267 _aServicio de Neurología-Neurofisiología
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3296719/ _yAcceso libre
942			_n0 _2ddc _cART
999			_c4556 _d4556