000			02263na a2200373 4500
999			_c4229 _d4229
003			PC4229
005			20210625062801.0
008			130622s2013 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aArenas Cortés, Alicia _92362 _eInstituto de Investigación i+12
100			_aAyala Díaz, Rosa María _91206 _eHematología y Hemoterapia
100			_aBarrio García, Santiago _91467 _eHematología y Hemoterapia
100			_aGallardo, Miguel _92437 _eHematología y Hemoterapia
100			_aGarcia Martin, Rosa Maria _92438 _eAnatomía Patológica
100			_aGilsanz Rodríguez, Florinda _91015 _eHematología y Hemoterapia
100			_aJiménez Ubieto, Ana Isabel _922 _eHematología y Hemoterapia
100			_aMartínez López, Joaquín _9389 _eHematología y Hemoterapia
100			_aToldos González, Oscar _91946 _eAnatomía Patológica
245	0	0	_aProteomic analysis reveals heat shock protein 70 has a key role in polycythemia Vera. _h[artículo]
260			_bMolecular cancer, _c2013
300			_a12:142.
500			_aFormato Vancouver: Gallardo M, Barrio S, Fernández M, Paradela A, Arenas A, Toldos O et al. Proteomic analysis reveals heat shock protein 70 has a key role in polycythemia Vera. Mol Cancer. 2013 Nov 19;12:142.
501			_aPMID: 24252366
504			_aContiene 35 referencias
520			_aJAK-STAT signaling through the JAK2V617F mutation is central to the pathogenesis of myeloproliferative neoplasms (MPN). However, other events could precede the JAK2 mutation. The aim of this study is to analyze the phenotypic divergence between polycytemia vera (PV) and essential thrombocytemia (ET) to find novel therapeutics targets by a proteomic and functional approach to identify alternative routes to JAK2 activation. Through 2D-DIGE and mass spectrometry of granulocyte protein from 20 MPN samples, showed differential expression of HSP70 in PV and ET besides other 60 proteins. Immunohistochemistry of 46 MPN bone marrow samples confirmed HSP70 expression. The median of positive granulocytes was 80% in PV (SD 35%) vs. 23% in ET (SD 34.25%). In an ex vivo model KNK437 was used as an inhibition model assay of HSP70, showed dose-dependent inhibition of cell growth and burst formation unit erythroid (BFU-E) in PV and ET, increased apoptosis in the erythroid lineage, and decreased pJAK2 signaling, as well as a specific siRNA for HSP70. These data suggest a key role for HSP70 in proliferation and survival of the erythroid lineage in PV, and may represent a potential therapeutic target in MPN, especially in PV.
710			_9330 _aServicio de Anatomía Patológica
710			_9297 _aServicio de Hematología y Hemoterapia
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/4/pc4229.pdf _ySolicitar documento
942			_n0 _2ddc _cART