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_c2892 _d2892 |
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003 | PC2892 | ||
005 | 20210625062759.0 | ||
008 | 130622s2013 xxx||||| |||| 00| 0 eng d | ||
040 | _cH12O | ||
041 | _aeng | ||
100 |
_aClemente, Paula _92400 _eInstituto de Investigación i+12 |
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_aCruz Bermúdez, Alberto _92401 _eInstituto de Investigación i+12 |
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_9862 _aGaresse, Rafael _eInstituto de Investigación i+12 |
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100 |
_aHernández Sierra, Rosana _92143 _eInstituto de Investigación I+12 |
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100 |
_aVallejo, Carmen G _92399 _eInstituto de Investigación i+12 |
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245 | 0 | 0 |
_aEvaluation of mitochondrial function and metabolic reprogramming during tumor progression in a cell model of skin carcinogenesis. _h[artículo] |
260 |
_bBiochimie, _c2013 |
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300 | _a95(6):1171-6. | ||
500 | _aFormato Vancouver: Vallejo CG, Cruz-Bermúdez A, Clemente P, Hernández-Sierra R, Garesse R, Quintanilla M. Evaluation of mitochondrial function and metabolic reprogramming during tumor progression in a cell model of skin carcinogenesis. Biochimie. 2013 Jun;95(6):1171-6. | ||
501 | _aPMID: 23353111 | ||
504 | _aContiene 21 referencias | ||
520 | _aMetabolic reprogramming from mitochondrial aerobic respiration to aerobic glycolysis is a hallmark of cancer. However, whether it is caused by a dysfunction in the oxidative phosphorylation pathway is still under debate. In this work, we have analyzed the bioenergetic cellular (BEC) index and the relative cell ability to grow in the presence of either galactose or glucose as sources of sugar (Gal/Glu index) of a system formed by four epidermal cell lines with increasing tumorigenic potentials, ranging from nontumorigenic to highly malignant. We find that the BEC index gradually decreases whereas the Gal/Glu index increases with tumorigenicity, indicating that a progressive metabolic adaptation to aerobic glycolysis occurs in tumor cells associated with malignancy. Interestingly, this metabolic adaptation does not appear to be caused by damaged respiration, since the expression and activity of components of the respiratory chain complexes were unchanged in the cell lines. Moreover, the corresponding mitochondrial ATP synthetic abilities of the cell lines were found similar. The production of reactive oxygen species was also measured. A shift in ROS generation was found when compared nontumorigenic with tumorigenic cell lines, the latter exhibiting about threefold higher ROS levels than nontumorigenic cells. This result indicates that oxidative stress is an early event during tumor progression. | ||
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_9625 _aInstituto de Investigación imas12 |
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_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/2/pc2892.pdf _ySolicitar documento |
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