000			02536na a2200337 4500
003			PC2787
005			20210625062758.0
008			130622s2012 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aCastillo Rama, Marcela _92127 _eInmunología
100			_aCastro Panete, María José _92010 _eInmunología
100			_aMeneu Díaz, Juan Carlos _91087 _eCirugía General y del Aparato Digestivo
100			_aMora Diaz, Sergio _91986 _eInmunología
100			_9393 _aMorales Pérez, Pablo _eInmunología
100			_9510 _aMoreno González, Enrique _eCirugía General y del Aparato Digestivo
100			_aPaz Artal, Estela _91510 _eInmunología
100			_aTalayero Giménez de Azcárate, Paloma _91511 _eInmunología
100			_aValero Hervás, Diana María _92129 _eInmunología
245	0	0	_aComplement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. _h[artículo]
260			_c2012 _bEuropean Journal of Inflammation,
300			_a10(3):329-34.
500			_aFormato Vancouver: Valero-Hervás DM, Morales P, Castro MJ, Varela P, Castillo-Rama M, Moreno E et al. Complement C3 genotyping of slow and fast variants by real time PCR-high resolution melting. Eur J Inflamm. 2012;10(3):329-34.
504			_aContiene 16 referencias
520			_a"Slow" and "Fast" C3 complement variants (C3S and C3F) result from a g.304C>G polymorphism that changes arginine to glycine at position 102. C3 variants are associated with complement-mediated diseases and outcome in transplantation. In this work C3 genotyping is achieved by a Real Time PCR - High Resolution Melting (RT-PCR-HRM) optimized method. In an analysis of 49 subjects, 10.2% were C3FF, 36.7% were C3SF and 53.1% were C3SS. AUelic frequencies (70% for C3S and 30% for C3F) were in Hardy-Weinberg equilibrium and similar to those published previously. When comparing RT-PCR-HRM with the currently used Tetraprimer-Amplification Refractory Mutation System PCR (T-ARMS-PCR), coincidence was 93.8"/o. The procedure shown here includes a single primer pair and low DNA amount per reaction. Detection of C3 variants by RT-PCR-HRM is accurate, easy, fast and low cost, and it may be the method of choice for C3 genotyping.
710			_9395 _aServicio de Inmunología
710			_9271 _aServicio de Cirugía General y del Aparato Digestivo
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/2/pc2787.pdf _ySolicitar documento
942			_n0 _2ddc _cART
999			_c2787 _d2787