000			01669na a2200229 4500
999			_c2024 _d2024
003			PC2024
005			20210625062757.0
008			130622s2013 xxx||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_aHernández Sierra, Rosana _92143 _eInstituto de Investigación I+12
100			_aGaresse, Rafael _9862 _eInstituto de Investigación i+12
100			_aMartinez Azorin, Francisco _92420 _eInstituto de Investigación i+12
245	0	0	_aMuscle-specific overexpression of the catalytic subunit of DNA polymerase γ induces pupal lethality in Drosophila melanogaster. _h[artículo]
260			_bArchives of Insect Biochemistry and physiology, _c2013
300			_a83(3):127-37.
500			_aFormato Vancouver: Martínez-Azorín F, Calleja M, Hernández-Sierra R, Farr CL, Kaguni LS, Garesse R. Muscle-specific overexpression of the catalytic subunit of DNA polymerase γ induces pupal lethality in Drosophila melanogaster. Arch Insect Biochem Physiol. 2013 Jul;83(3):127-37.
501			_aPMID:23729397
504			_aContiene 33 referencias
520			_aWe show the physiological effects and molecular characterization of overexpression of the catalytic core of mitochondrial DNA (mtDNA) polymerase (pol -) in muscle of Drosophila melanogaster. Muscle-specific overexpression of pol - using the UAS/GAL4 (where UAS is upstream activation sequence) system produced more than 90% of lethality at the end of pupal stage at 25 degrees C, and the survivor adult flies showed a significant reduction in life span. The survivor flies displayed a decreased mtDNA level that is accompanied by a corresponding decrease in the levels of the nucleoid-binding protein mitochondrial transcription factor A (mtTFA). Furthermore, an increase in apoptosis is detected in larvae and adults overexpressing pol -. We suggest that the pupal lethality and reduced life span of survivor adult flies are both caused mainly by massive apoptosis of muscle cells induced by mtDNA depletion.
710			_9625 _aInstituto de Investigación imas12
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4703106/pdf/nihms747235.pdf _yAcceso libre
942			_n0 _2ddc _cART