000			nab a22 7a 4500
999			_c17999 _d17999
003			PC17999
005			20250617110033.0
008			250617b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_92081 _aBarcena García, Carmen _eAnatomía Patológica
245	0	0	_aMesothelial-to-mesenchymal transition in the pathogenesis of post-surgical peritoneal adhesions. _h[artículo]
260			_bThe Journal of pathology, _c2016
300			_a239(1):48-59. .
500			_aFormato Vancouver: Sandoval P, Jiménez Heffernan JA, Guerra Azcona G, Pérez Lozano ML, Rynne Vidal Á, Albar Vizcaíno P et al. Mesothelial-to-mesenchymal transition in the pathogenesis of post-surgical peritoneal adhesions. J Pathol. 2016 May;239(1):48-59.
501			_aPMID: 27071481
504			_aContiene 61 referencias
520			_aPeritoneal adhesions (PAs) are fibrotic bands formed between bowel loops, solid organs, and the parietal peritoneum, which may appear following surgery, infection or endometriosis. They represent an important health problem with no effective treatment. Mesothelial cells (MCs) line the peritoneal cavity and undergo a mesothelial-to-mesenchymal transition (MMT) under pathological conditions, transforming into myofibroblasts, which are abundant in peritoneal fibrotic tissue. The aim of this study was to investigate if peritoneal MCs undergo a MMT contributing to the formation of post-surgical adhesions. Biopsies from patients with PAs were analysed by immunohistochemistry, immunofluorescence, and quantitative RT-PCR. A mouse model of PAs based on ischaemic buttons was used to modulate MMT by blocking the transforming growth factor-beta (TGF-β) pathway. The severity of adhesions and MMT-related marker expression were studied. We observed myofibroblasts derived from the conversion of MCs in submesothelial areas of patients with PAs. In addition, MMT-related markers were dysregulated in adhesion zones when compared to distant normal peritoneal tissue of the same patient. In animal experiments, blockage of TGF-β resulted in molecular reprogramming of markers related to the mesenchymal conversion of MCs and in a significant decrease in the severity of the adhesions. These data indicate for the first time that MMT is involved in PA pathogenesis. This finding opens new therapeutic strategies to interfere with adhesion formation by modulating MMT with a wide range of pharmacological agents.
710			_9330 _aServicio de Anatomía Patológica
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc17999.pdf _ySolicitar documento
942			_2ddc _cART _n0