000			nab a22 7a 4500
999			_c17915 _d17915
003			PC17915
005			20241205123540.0
008			241202b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_93249 _aGómez Cerezo, N _eInstituto de Investigación imas12
100			_92647 _aSánchez Salcedo, Sandra _eInstituto de Investigación i+12
100			_93054 _aIzquierdo Barba, Isabel _eInstituto de Investigación imas12
100			_92843 _aArcos, Daniel _eInstituto de Investigación imas12
100			_92644 _aVallet Regí, María _eInstituto de Investigación imas12
245	0	0	_aIn vitro colonization of stratified bioactive scaffolds by pre-osteoblast cells. _h[artículo] _c
260			_bActa biomaterialia, _c2016
300			_a44:73-84.
500			_aFormato Vancouver: Gómez Cerezo N, Sánchez Salcedo S, Izquierdo Barba I, Arcos D, Vallet Regí M. In vitro colonization of stratified bioactive scaffolds by pre-osteoblast cells. Acta Biomater. 2016 Oct 15;44:73-84.
501			_aPMID: 27521495
504			_aContiene 77 referencias
520			_aMesoporous bioactive glass-polycaprolactone (MBG-PCL) scaffolds have been prepared by robocasting, a layer by layer rapid prototyping method, by stacking of individual strati. Each stratus was independently analyzed during the cell culture tests with MC3T3-E1 preosteblast-like cells. The presence of MBG stimulates the colonization of the scaffolds by increasing the cell proliferation and differentiation. MBG-PCL composites not only enhanced pre-osteoblast functions but also allowed cell movement along its surface, reaching the upper stratus faster than in pure PCL scaffolds. The cells behavior on each individual stratus revealed that the scaffolds colonization depends on the chemical stimuli supplied by the MBG dissolution and surface changes associated to the apatite-like formation during the bioactive process. Finally, scanning electron and fluorescence microscopy revealed that the kinetic of cell migration strongly depends on the architectural features of the scaffolds, in such a way that layers interconnections are used as migration routes to reach the farther scaffolds locations from the initial cells source. Statement of significance: This manuscript provides new insights on cell behavior in bioceramic/polymer macroporous scaffolds prepared by rapid prototyping methods. The experiments proposed in this work have allowed the evaluation of cell behavior within the different levels of the scaffolds, i.e. from the initials source of cells towards the farther scaffold locations. We could demonstrate that the in vitro cell colonization is encouraged by the presence of a highly bioactive mesoporous glass (MBG). This bioceramic enhances the cell migration towards upper strati through the dissolution of chemical signals and the changes occurred on the scaffolds surface during the bioactive process. In addition the MBG promotes preosteblastic proliferation and differentiation respect to scaffolds made of pure polycaprolactone. Finally, this study reveals the significance of the architectural design to accelerate the cell colonization. These experiments put light on the factors that should be taken into account to accelerate the regeneration processes under in vivo conditions.
710			_9625 _aInstituto de Investigación imas12
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc17915.pdf _ySolicitar documento
942			_2ddc _cART _n0