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999			_c17903 _d17903
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005			20240711141208.0
008			240711b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_93304 _aMolina Pinelo, Sonia _eOncología Médica
100			_93364 _aFerrer, Irene _eInstituto de Investigación imas12
100			_93421 _aSuárez Beltrán, Rocío _eOncología Médica
100			_91811 _aPaz-Ares Rodríguez, Luis _eOncología Médica
245	0	0	_aImpact of DLK1-DIO3 imprinted cluster hypomethylation in smoker patients with lung cancer. _h[artículo]
260			_bOncotarget, _c2016
300			_a9(4):4395-410.
500			_aFormato Vancouver: Molina Pinelo S, Salinas A, Moreno Mata N, Ferrer I, Suarez R, Andrés León E et al. Impact of DLK1-DIO3 imprinted cluster hypomethylation in smoker patients with lung cancer. Oncotarget. 2016 Jul 15;9(4):4395-410.
501			_aPMID: 29435111 PMC5796982
504			_aContiene 60 referencias
520			_aDNA methylation is important for gene expression and genome stability, and its disruption is thought to play a key role in the initiation and progression of cancer and other diseases. The DLK1-DIO3 cluster has been shown to be imprinted in humans, and some of its components are relevant to diverse pathological processes. The purpose of this study was to assess the methylation patterns of the DLK1-DIO3 cluster in patients with lung cancer to study its relevance in the pathogenesis of this disease. We found a characteristic methylation pattern of this cluster in smoking associated lung cancer, as compared to normal lung tissue. This methylation profile is not patent however in lung cancer of never smokers nor in lung tissue of COPD patients. We found 3 deregulated protein-coding genes at this locus: one was hypermethylated (DIO3) and two were hypomethylated (DLK1 and RTL1). Statistically significant differences were also detected in two different families of SNORDs, two miRNA clusters and four lncRNAs (MEG3, MEG8, MEG9 and LINC00524). These findings were validated using data from the cancer genome atlas (TCGA) database. We have then showed an inverse correlation between DNA methylation and expression levels in 5 randomly selected genes. Several targets of miRNAs included in the DLK1-DIO3 cluster have been experimentally verified as tumor suppressors. All of these results suggest that the dysmethylation of the imprinted DLK1-DIO3 cluster could have a relevant role in the pathogenesis of lung cancer in current and former smokers and may be used for diagnostic and/or therapeutic purposes.
710			_9303 _aServicio de Oncología Médica
710			_9625 _aInstituto de Investigación imas12
856			_uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC5796982/pdf/oncotarget-09-4395.pdf _yAcceso libre
942			_2ddc _cART _n0