000			nab a22 7a 4500
999			_c17614 _d17614
003			PC17614
005			20230725135534.0
008			230725b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_92596 _aAlfaro, Javier _eInstituto de Investigación i+12
100			_92937 _aPérez Méndez, Dolores _eInmunología
100			_9358 _aJiménez Romero, Carlos _eCirugía General y del Aparato Digestivo
100			_92597 _aMartinez-Flores, Jose A. _eInstituto de Investigación i+12
100			_91105 _aGrau Sanz, Montserrat _eCirugía General y del Aparato Digestivo
100			_92462 _aSánchez Zapardiel, Elena _eInmunología
100			_91510 _aPaz Artal, Estela _eInmunología
100			_9661 _aSerrano Hernández, Antonio _eInmunología
245	0	0	_aBlockade of cell adhesion molecules enhances cell engraftment in a murine model of liver cell transplantation. _h[artículo]
260			_bTransplant immunology, _c2016
300			_a35:7-11.
500			_aFormato Vancouver: Alfaro J, Pérez D, Jiménez C, Serrano M, Martínez Flores JÁ, Grau M et al. Blockade of cell adhesion molecules enhances cell engraftment in a murine model of liver cell transplantation. Transpl Immunol. 2016 Mar;35:7-11.
501			_aPMID: 26875547
504			_aContiene 34 referencias
520			_aAim: OLT is the best alternative for patients with end-stage liver diseases. However, as the need for organs surpasses donor availability, alternatives to OLT are required. LCT could be a useful option versus OLT in several patients even though its low cell-engraftment hampers its efficiency. Endothelial cell barrier is the main obstacle for the implantation of cells into the parenchyma. Our study has focused on the modification of the endothelial barrier with monoclonal antibodies against adhesion molecules in order to increase cell engraftment in a mouse model of liver cell transplantation. Methods: Anti-mouse CD54 and anti-mouse CD61 antibodies were administered intrasplenically to healthy mice within 60 min prior to stem cell transplantation. Animals were sacrificed either short term at 2h or middle term seven days after transplantation. Immunohistochemical techniques to detect alkaline phosphatase activity were used to identify the transplanted cells within the liver parenchyma. Results: Anti-CD54 and anti-CD61 administration increases vascular patency and cell engraftment. This represents a 32% and 45% increase, respectively, of engrafted cells compared to the control (p<0.05). Conclusion: Modification of the vascular wall with monoclonal antibodies against endothelial adhesion molecules before cell transplantation enhances cell engraftment into the mouse liver.
710			_9625 _aInstituto de Investigación imas12
710			_9395 _aServicio de Inmunología
710			_9271 _aServicio de Cirugía General y del Aparato Digestivo
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc17614.pdf _ySolicitar documento
942			_2ddc _cART _n0