000			nab a22 4500
999			_c15991 _d15991
003			PC15991
005			20210625062817.0
008			200615b xxu||||| |||| 00| 0 eng d
040			_cH12O
041			_aeng
100			_92597 _aMartinez-Flores, Jose A. _eInstituto de Investigación i+12
100			_92598 _aSerrano, Manuel _eInstituto de Investigación i+12
100			_9393 _aMorales Pérez, Pablo _eInmunología
100			_91510 _aPaz Artal, Estela _eInmunología
100			_9576 _aMorales Cerdán, José María _eNefrología
100			_9661 _aSerrano Hernández, Antonio _eInmunología
245	0	0	_aComparison of several functional methods to evaluate the immune response on stable kidney transplant patients. _h[artículo]
260			_bJournal of immunological methods, _c2014
300			_a403(1-2):62-5.
500			_aFormato Vancouver: Martínez-Flores JA, Serrano M, Morales P, Paz-Artal E, Morales JM, Serrano A. Comparison of several functional methods to evaluate the immune response on stable kidney transplant patients. J Immunol Methods. 2014 Jan 31;403(1-2):62-5.
501			_aPMID: 24291342
504			_aContiene 23 referencias
520			_a The introduction of new immunosuppressive drugs in the last two decades has been associated with a significant decline in the prevalence of acute rejection and a huge improvement of graft survival. Monitoring blood levels of immunosuppressive drugs is the most common way to control drug doses in renal transplant patients. This approach is useful and widely used but doesn't give accurate information about the immune status of the patient. For this goal, there are many "in house" protocols which give more information, but cannot be standardized, limiting their applicability to compare results between different laboratories. In this study we compare three classical functional methods to evaluate the immune response: Mixed lymphocyte reaction (MLR), phytohemagglutinin stimulated peripheral blood lymphocytes (PBLs), and anti-CD3 monoclonal antibodies (mAbs) against PBL with the only FDA-labeled assay to measure the patient immune status: Cylex ImmuKnow® that measures the intracelullar ATP in CD4+ lymphocytes. We used n=111 stable renal transplant patients, all the patients with more than one year functioning grafts. We referred the results to a control population of healthy blood donors (n=125). Results: Measurement of intracellular ATP in CD4+ lymphocytes is able to differentiate immunosuppressed populations in renal transplant patients from health controls (242.30±21.62 vs. 386.43±25.12, p 0.0001). By contrary, there were no differences between controls and renal recipients when functional response was measured by MLR, PHA and anti-CD3 mAbs (2.48±0.45 vs. 2.37±0.41; 2.84±0.76 vs. 2.37±0.32; 2.32±0.34 vs. 1.89±0.38 respectively). In summary, our results show that the measurement of ATP in CD4+ lymphocytes gives more accurate information in comparison to the classical methods.
710			_9625 _aInstituto de Investigación imas12
710			_9395 _aServicio de Inmunología
710			_986 _aServicio de Nefrología
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc15991.pdf _ySolicitar documento
942			_2ddc _cART _n0