000			02634na a2200277 4500
999			_c12944 _d12944
003			PC12944
005			20181106130032.0
008			130622s2013 xxx||||| |||| 00| 0 eng d
024			_a10.1097/MPG.0b013e31828393ed
040			_cH12O
041			_aeng
100			_aBustos Lozano, Gerardo _91378 _eNeonatología
100			_aEscuder Vieco, Diana _91856 _b _eNeonatología
100			_aPallás Alonso, Carmen Rosa _91008 _eNeonatología
245	0	0	_aBacteriological, Biochemical, and Immunological Modifications in Human Colostrum After Holder Pasteurisation. _h[artículo]
260			_bJournal of Pediatric Gastroenterology and Nutrition, _c2013
300			_a56(5):560-8.
500			_aFormato Vancouver: Espinosa-Martos I, Montilla A, de Segura AG, Escuder D, Bustos G, Pallás C et al. Bacteriological, biochemical, and immunological modifications in human colostrum after Holder pasteurisation. J Pediatr Gastroenterol Nutr. 2013 May;56(5):560-8.
501			_aPMID: 23274339
504			_aContiene 50 referencias
520			_aObjective: The objective of this work was to evaluate the effect of Holder pasteurisation of human colostrum on a variety of microbiological, biochemical, and immunological parameters. Methods: Colostrum samples from 10 donors, and 8 samples of mature milk used as controls, were heated at 62.5 degrees C for 30 minutes. Bacterial counts and the concentration of furosine, lactose, myoinositol, glucose, lactulose, cytokines, and immunoglobulins were determined before and after the heat treatment. Results: Mean bacterial counts in nonpasteurised colostrum samples oscillated between 2.72 and 4.13 log(10) colony-forming units per millilitre in the agar media tested. Holder pasteurisation led to the destruction of the bacteria originally present in the samples. Furosine was detected in all samples before pasteurisation and increased significantly after the heat treatment (from 6.60 to 20.59 mg/100 g protein). Lactulose content was below the detection limit in nonpasteurised colostrum, but it was detected in all samples and quantified in 7 of them (from 10.68 to 38.02 mg/L) after Holder pasteurisation. Lactose, glucose, and myoinositol concentrations did not change after Holder pasteurisation. The concentrations of most cytokines and immunoglobulins were significantly higher in colostrum than in mature milk samples. Immunoglobulin content, both in colostrum and in milk samples, was reduced during pasteurisation, whereas, among cytokines, only macrophage inflammatory protein-1 beta, interleukin-7, and granulocyte-macrophage-colony-stimulating factor concentrations were affected by this heat treatment. Conclusions: Lactulose and furosine content could be used as heat treatment indicators in colostrum samples. Holder pasteurisation modified the immunological profile of both colostrum and mature milk.
710			_9446 _aServicio de Pediatría-Neonatología
856			_uhttp://pc-h12o-es.m-hdoct.a17.csinet.es/pdf/pc/1/pc12944.pdf _ySolicitar documento
942			_n0 _2ddc _cART