Progenitor-derived endothelial cell response, platelet reactivity and haemocompatibility parameters indicate the potential of NaOH-treated polycaprolactone for vascular tissue engineering [artículo]
Por: Comas Illas, Juan Valentín [Cirugía Cardíaca Pediátrica].
Colaborador(es): Servicio de Pediatría-Neonatología.
Editor: Journal of Tissue Engineering and Regenerative Medicine, 2011Descripción: 5(3):238-247.Recursos en línea: Solicitar documento Resumen: The haemocompatibility of NaOH-treated poly(e-caprolactone) (PCL) has been evaluated in vitro by analysing several parameters, including plasma recalcification time, whole blood clotting time and platelet adhesion/activation. NaOH-treated PCL films showed a significant decrease in the clot formation speed and a reduced number of adhered platelets, which mainly exhibited non-activated morphologies. Furthermore, mature endothelial cells derived from peripheral endothelial progenitor cells were cultured on the polymer to investigate the effects of the endothelial lining on polymer haemocompatibility. Interestingly, cells cultured on NaOH-treated PCL films showed a significant stimulation of NO production. Although further research is required, NaOH treatment could be an interesting and simple strategy to modify PCL-based materials in order to enhance endothelial NO production, where compromised, and provide a better interaction of the scaffold with the blood components. In conclusion, these results reinforce the use of NaOH-treated PCL as a haemocompatible polymer for vascular tissue-engineering applications.Tipo de ítem | Ubicación actual | Signatura | Estado | Fecha de vencimiento |
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Artículo | PC8302 (Navegar estantería) | Disponible |
Formato Vancouver:
Serrano MC, Pagani R, Peña J, Vallet-Regí M, Comas JV, Portolés MT. Progenitor-derived endothelial cell response, platelet reactivity and haemocompatibility parameters indicate the potential of NaOH-treated polycaprolactone for vascular tissue engineering. J Tissue Eng Regen Med. 2011;5(3):238-47.
PMID: 20687124
Contiene 42 referencias
The haemocompatibility of NaOH-treated poly(e-caprolactone) (PCL) has been evaluated in vitro by analysing several parameters, including plasma recalcification time, whole blood clotting time and platelet adhesion/activation. NaOH-treated PCL films showed a significant decrease in the clot formation speed and a reduced number of adhered platelets, which mainly exhibited non-activated morphologies. Furthermore, mature endothelial cells derived from peripheral endothelial progenitor cells were cultured on the polymer to investigate the effects of the endothelial lining on polymer haemocompatibility. Interestingly, cells cultured on NaOH-treated PCL films showed a significant stimulation of NO production. Although further research is required, NaOH treatment could be an interesting and simple strategy to modify PCL-based materials in order to enhance endothelial NO production, where compromised, and provide a better interaction of the scaffold with the blood components. In conclusion, these results reinforce the use of NaOH-treated PCL as a haemocompatible polymer for vascular tissue-engineering applications.
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